CPICS Submersible Microscope

About the CPICS Submersible Microscope

CPICS is a submersible microscope that captures sharp, high-resolution images of plankton and suspended particles directly in the water. It uses darkfield lighting so the camera mainly sees light scattered by the organism or particle, which makes fine features stand out. The imaging space is open and flow-through, so fragile targets can pass through naturally and stay intact. The system can also sort and label images automatically using image-quality checks, in-focus detection, and trained categories.
Core Technology
TBD

CPICS can capture small organisms and particles with high clarity by using darkfield illumination that highlights fine structure. It can image targets without trapping or pumping because water flows through an open imaging space. It can produce consistent size measurements because telecentric optics maintain stable magnification across the focal region. It can reduce data overload by saving only the cropped regions of interest rather than every full frame. It can support longer deployments using anti-fouling options such as ultraviolet exposure, optical-surface treatments, or mechanical cleaning methods.

ParameterMeasured value
Smallest target size captured clearlyDown to ~10 µm targets with fine details down to ~1 µm
Imaging methodDarkfield illumination with light directed so it avoids the lens unless scattered by a target
Imaging spaceOpen flow-through target space so organisms pass through naturally without collection
OpticsTelecentric lens for stable magnification and reduced distortion
Illumination geometryAngled beams that converge in the imaging space, with a documented configuration near 42° relative to the central imaging axis
Illumination timingStrobed or intermittent lighting synchronized to camera exposure to reduce motion blur
Deployment depthDesigned for submergence from shallow water to deep deployments up to ~6,000 m
Light-source standoff distanceDocumented configurations place the light source about 1 cm to 6 cm from the optical system
Imaging rateDocumented operating modes include ~4 frames per second, with other modes supporting higher frame rates
Imaged water volume per frameDocumented configuration images an ~8.00 × 7.50 × 0.55 mm water volume per frame
Processing outputsRegion-of-interest extraction, in-focus screening, and automated classification into trained categories
Anti-fouling optionsUltraviolet exposure, optical-surface treatments, mechanical cleaning methods, or combinations
  • Continuous plankton and particle monitoring on moorings, buoys, and subsea observatories
  • Time-series tracking of community shifts during storms, blooms, and mixing events
  • Imaging fragile organisms and aggregates such as gelatinous plankton and marine snow
  • Vehicle-based surveys on profilers, towed bodies, ROVs, AUVs, and gliders
  • Automated counts and size distributions for ecosystem indicators and research datasets

TRL 6

The system has been demonstrated as a submersible prototype on an underwater observatory node about 1.6 m above the seafloor, continuously imaging an approximately 8.00 × 7.50 × 0.55 mm volume of water at about 4 frames per second while extracting and saving in-focus regions of interest for automated classification.

About the CPICS Submersible Microscope

Overview

CPICS is a submersible microscope that captures sharp, high-resolution images of plankton and suspended particles directly in the water. It uses darkfield lighting so the camera mainly sees light scattered by the organism or particle, which makes fine features stand out. The imaging space is open and flow-through, so fragile targets can pass through naturally and stay intact. The system can also sort and label images automatically using image-quality checks, in-focus detection, and trained categories.
Intellectual Property

The system forms a defined imaging space in front of a camera and telecentric lens and illuminates that space with angled light that does not shine straight into the lens. When an organism or particle enters the imaging space, it scatters the light toward the lens, producing a darkfield image with strong edge and texture detail. Software then detects targets in each frame, crops each target into a region of interest, rejects out-of-focus targets using a focus metric, and classifies the remaining images using trained taxonomic or particle categories.

Traditional plankton sampling often relies on nets or bottles, which can break fragile organisms, mix samples across depth, and miss short-lived events. Many imaging systems also struggle to keep targets in focus and to classify large numbers of images reliably. CPICS addresses these limits by imaging continuously in situ, keeping the imaging volume open so organisms are not squeezed through a chamber, and using automated focus screening and classification so high-volume datasets become usable without manual sorting.

CPICS can capture small organisms and particles with high clarity by using darkfield illumination that highlights fine structure. It can image targets without trapping or pumping because water flows through an open imaging space. It can produce consistent size measurements because telecentric optics maintain stable magnification across the focal region. It can reduce data overload by saving only the cropped regions of interest rather than every full frame. It can support longer deployments using anti-fouling options such as ultraviolet exposure, optical-surface treatments, or mechanical cleaning methods.

ParameterMeasured value
Smallest target size captured clearlyDown to ~10 µm targets with fine details down to ~1 µm
Imaging methodDarkfield illumination with light directed so it avoids the lens unless scattered by a target
Imaging spaceOpen flow-through target space so organisms pass through naturally without collection
OpticsTelecentric lens for stable magnification and reduced distortion
Illumination geometryAngled beams that converge in the imaging space, with a documented configuration near 42° relative to the central imaging axis
Illumination timingStrobed or intermittent lighting synchronized to camera exposure to reduce motion blur
Deployment depthDesigned for submergence from shallow water to deep deployments up to ~6,000 m
Light-source standoff distanceDocumented configurations place the light source about 1 cm to 6 cm from the optical system
Imaging rateDocumented operating modes include ~4 frames per second, with other modes supporting higher frame rates
Imaged water volume per frameDocumented configuration images an ~8.00 × 7.50 × 0.55 mm water volume per frame
Processing outputsRegion-of-interest extraction, in-focus screening, and automated classification into trained categories
Anti-fouling optionsUltraviolet exposure, optical-surface treatments, mechanical cleaning methods, or combinations
  • Continuous plankton and particle monitoring on moorings, buoys, and subsea observatories
  • Time-series tracking of community shifts during storms, blooms, and mixing events
  • Imaging fragile organisms and aggregates such as gelatinous plankton and marine snow
  • Vehicle-based surveys on profilers, towed bodies, ROVs, AUVs, and gliders
  • Automated counts and size distributions for ecosystem indicators and research datasets

TRL 6

The system has been demonstrated as a submersible prototype on an underwater observatory node about 1.6 m above the seafloor, continuously imaging an approximately 8.00 × 7.50 × 0.55 mm volume of water at about 4 frames per second while extracting and saving in-focus regions of interest for automated classification.

Case Number

WHOI-OW-571

Patent

US 10222688 B2

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